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Thursday, July 30, 2020 | History

2 edition of three-dimensional structure of asparagine-linked glycopeptides. found in the catalog.

three-dimensional structure of asparagine-linked glycopeptides.

Jean-Robert Brisson

three-dimensional structure of asparagine-linked glycopeptides.

by Jean-Robert Brisson

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  • 17 Currently reading

Published .
Written in English


The Physical Object
Pagination196, [13, 41] leaves.
Number of Pages196
ID Numbers
Open LibraryOL14720155M

Galectin-1, a β-galactoside binding lectin involved in immunoregulation and cancer, binds natural and many synthetic multivalent glycoconjugates with an apparent glycoside cluster effect, that is, affinity above and beyond what would be expected from the concentration of the determinant sugar. Here we have analyzed the mechanism of such cluster effects in solution at . Sali A; Matsumoto R; McNeil HP; Karplus M; Stevens RL, , 'Three-dimensional models of four mouse mast cell chymases. Identification of proteoglycan binding regions and protease-specific antigenic epitopes', Journal of Biological Chemistry, vol. , pp. -

Glycosylation Last updated Septem Glycosylation (see also chemical glycosylation) is the reaction in which a carbohydrate, i.e. a glycosyl donor, is attached to a hydroxyl or other functional group of another molecule (a glycosyl acceptor).In biology, glycosylation mainly refers in particular to the enzymatic process that attaches glycans to proteins, or other organic . The book, whose contributors are largely drawn from industry, is primarily aimed at an industrial audience. However, it should also prove a useful reference source to research and educational personnel with a direct interest in t h t s field. 1x. Biopharmace uticals, an ove wiew.

Glycoprotein De-N-glycosylation with PNGase F.- MALDI-MS of glycoproteins.- Analysis of Glycopeptides.- Structural analysis of N-glycans by MALDI-MS of glycopeptides in combination with exoglycosidase array sequencing.- Accessing the complex carbohydrate structure database.- References.- 15 The Application of Three-Dimensional HPLC. One synthetic route to glycopeptides involves the use of b-glycosylamines as intermedi- ates, these then being reacted with a suitably protected amino acid or polypeptide side chain. 4 1 The b -glycosylamines can be prepared directly from a fully deprotected sugar by treatment with 40–50 equivalents of ammonium bicarbonate at room temperature.


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Three-dimensional structure of asparagine-linked glycopeptides by Jean-Robert Brisson Download PDF EPUB FB2

The asparagine-linked oligosaccharides represent only one class of covalently bound carbohydrates found on glycoproteins, but within this class there are many structural variations. 14,15 They have been classified into three general types: the high-mannose, the complex, and the hybrid N-glycans, as depicted in Figure N-glycans have the same basic pentasaccharide core structure.

Site-directed mutagenesis of the two asparagine-linked glycosylation sites of hCG alpha was used to study the function of the individual oligosaccharide chains in secretion and subunit assembly.

Expression vectors for the alpha genes (wild-type and mutant) and the hCG beta gene were constructed and transfected into Chinese hamster ovary by: structure of oligosaccharide A-2 is proposed as shown in Fig.

that three different glycopeptides are found in human. asparagine-linked sugar chains are. This convergent approach for the chemical synthesis of asparagine-linked glycopeptides relies on the synthesis of protected peptides with orthogonally protected aspartic acid side chain, which upon removal permits activation and the coupling of glycosylamine (Scheme 3).

52 The major limitation of this approach is the size of the used protected. Primary and Three-Dimensional Structure of Lactotransferrin (Lactoferrin) Glycans Kobata A () Structural study of the sugar chains of human lactotransferrins: finding of four novel complex-type asparagine-linked sugar chains.

Bayard B, Charet P, Bouquelet S, Strecker G, Montreuil J, () Structure of glycopeptides isolated from Cited by: After pronase digestion of these glycoproteins, the 35S-label remained associated with the glycopeptides three-dimensional structure of asparagine-linked glycopeptides.

book on asparagine-linked carbohydrate units which were released by. Figure 6: Structure of the E. coli MsbA dimer at Å resolution. This backbone tracing was made from protein data bank file 1JSQ (). Trans. Three-Dimensional Structure of S 3-RNase. A homology model for the three-dimensional structure of S 3-RNase from L.

peruvianum was constructed from the coordinates of RNase Rh (Kurihara et al., ) and this is shown in Figure 4. A sequence alignment of the two proteins shows that most of the differences occur in loop regions rather than in. Jean Robert Brisson, Ph.D. thesis: The Three-Dimensional Structure of Asparagine-Linked Glycopeptides, University of Toronto () Google Scholar Harry Schacter, Saroja Narasimhan, Noam Harpaz and Gregory D.

Longmore in: Membranes and Transport, ed. by Anthony N. Mastonosi, Plenum Pub. Corp. vol. 1, pp. – () Google Scholar. The ISOBM TD-7 Workshop on hCG and related molecules. Towards user-oriented standardization of pregnancy and tumor diagnosis: Assignment of epitopes to the three-dimensional structure of diagnostically and commercially relevant monoclonal antibodies directed against human chorionic gonadotropin and derivatives.

Tumor Biol. 23, 1– A branch of an oligosaccharide emanating from a “core” structure. Asparagine-linked oligosaccharide. A glycosylated amino acid used in solid-phase peptide synthesis to generate glycopeptides. A two-dimensional representation of a three-dimensional organic molecule devised by Hermann Emil Fischer.

Sequence and three-dimensional structure of griffithsin (GRFT). (A) Sequence of wild-type GRFT. Amino acids located within beta strands are colored red and the black arrows correspond to secondary structure.

X represents an unknown amino acid of mass present in the natural alga-derived material. Oligosaccharide epitopes are recognized through their three-dimensional structure and their flexibility is a key issue in specificity. In this paper, we analysed by X-ray crystallography the structures of the LecB lectin from two strains of Pseudomonas aeruginosa in complex with Lewis x oligosaccharide present on cell surfaces of human tissues.

N-glycosylation of eukaryotic proteins is widespread and vital to survival. The pentasaccharide unit −Man3GlcNAc2– lies at the protein-junction core of all oligosaccharides attached to asparagine side chains during this process.

Although its absolute conservation implies an indispensable role, associated perhaps with its structure, its unbiased conformation and the. An illustration of an open book.

Books. An illustration of two cells of a film strip. Video. An illustration of an audio speaker. Audio. An illustration of a " floppy disk.

Software. An illustration of two photographs. Images. An illustration of a heart shape Donate. An illustration of text ellipses. The present invention relates to Fc region-containing polypeptides that exhibit improved effector function due to alterations in the degree of fucosylation, and methods of using such polypeptides to treat or prevent cancer and other diseases.

The Fc region-containing polypeptide of the invention is preferably an immunoglobulin (such as an antibody), wherein the Fc region. Genes Dev ;– Merkle RK, Cummings RD. Relationship of the terminal sequences to the length of poly-Nacetyllactosamine chains in asparagine-linked oligosaccharides from the mouse lymphoma cell line BW Immobilized tomato lectin interacts with high affinity with glycopeptides containing long poly-N-acetyllactosamine chains.

Carrer JP, Brisson JR () The three dimensional structure of N-linked oligosaccharides. In: Ginsburg V, Robbins PW (eds) The Biology of Carbohydrates, Vol. Wiley New York, pp – Google Scholar. Other substitutions leading to elimination of asparagine-linked glycosylation at N include modifications at residues T (or S together with T), see, e.g., any of the mutations and combinations of mutations disclosed in Sazinsky et al.

Proc. Nat. Acad. Sci. U.S.A. (51), the disclosure of which is incorporated by. This book features hundreds of passages from Greek and Roman authors, with gentle guidance from McKeown, giving a vividly direct picture of the ancient medical world, a world in which, for example, a surgeon had to be strong-minded enough to ignore the screams of his patient, diseases were assumed to be sent by the gods, medicine and magic were.

Analysis of Three Epoetin Alpha Products by LC and LC-MS Indicates Differences in Glycosylation Critical Quality Attributes, Including Sialic Acid Content. Search metadata Search text contents Search TV news captions Search radio transcripts Search archived web sites Advanced Search.The structure of the PA-sugar chain was determined by two-dimensional sugar mapping combined with sequential exoglycosidase digestion and partial acid hydrolysis, and by MHz 1H-NMR spectroscopy.

The structure found was Manalpha(Manalpha)Manalpha(Manalpha) (Xylbeta)Manbeta1- 4GlcNAcbeta(Fucalpha)GlcNAc.